

Some CC can cause issues with the rigs later on in Blender. If you don’t know how to make poses, this tutorial will be too advanced for you.īefore you start this process at all, it’s recommended that you go in-game and remove any CC on your Sim that isn’t needed for the pose you’re making. I am only focusing on creating custom rigs for the purposes of pose creation. This is not a tutorial on how to make poses or how to use Blender.

Together, these findings highlight SIM as a powerful tool to advance CESA imaging beyond the resolution limit of conventional light microscopy.If you’re a pose creator, you’ll understand the struggle of creating a pose for your story only to have it not fit your Sims right because they have a smaller or larger body type than the default EA rigs you used in Blender. CESA track patterns can vary widely between neighboring cells of similar shape, implying that cellulose patterning is not the sole determinant of cellular growth anisotropy. SIM data also reveal that CESA particles vary in their overlaps with microtubule tracks and can complete U-turns without changing speed. CESA particles tracked by SIM display minimal variation in velocity, suggesting coordinated control of CESA catalytic activity within single complexes and that CESA complexes might move steadily in tandem to generate larger cellulose fibrils or bundles. SIM imaging reveals that Arabidopsis thaliana CESA particles are more than twice as dense in the plasma membrane as previously estimated, helping explain the dense arrangement of cellulose observed in new wall layers.

Here, we applied Structured Illumination Microscopy (SIM), which improves resolution two-fold over confocal or widefield imaging, to explore the dynamic behaviors of CESA particles in living plant cells. However, the resolution limit of confocal microscopy circumscribes what can be discovered about these tiny biosynthetic machines. Confocal imaging has shown that CELLULOSE SYNTHASE (CESA) particles move through the plasma membrane as they synthesize cellulose.
